1. Light Availability:

Light is a crucial factor for chlorophyll synthesis. Plants require adequate sunlight for optimal photosynthesis. In low-light conditions, chlorophyll production can be inhibited, leading plants to develop elongated stems in search of light (a phenomenon known as etiolation) and reducing overall chlorophyll levels.

2. Nutrient Availability:

Chlorophyll synthesis is heavily influenced by the availability of certain nutrients, particularly nitrogen, magnesium, and iron. Nitrogen is a key component of chlorophyll molecules, and a deficiency can lead to yellowing of leaves, known as chlorosis. Magnesium acts as a central atom in the chlorophyll molecule, and iron is essential for the enzymatic processes involved in chlorophyll production.

3. Water Supply:

Water is integral to plant health and photosynthesis. Insufficient water can lead to stress, which may inhibit chlorophyll production. Conversely, overwatering can lead to root damage and poor nutrient uptake, also affecting chlorophyll levels.

4. Temperature:

Temperature has a significant impact on enzymatic activity related to chlorophyll synthesis. Optimal temperatures promote robust chlorophyll production, while extreme temperatures can inhibit this process. For example, high temperatures can cause protein denaturation and reduce chlorophyll concentration.

5. Carbon Dioxide Levels: Carbon dioxide is a critical raw material for photosynthesis. Higher concentrations of CO2 can enhance photosynthesis rates, which may lead to increased chlorophyll production. Conversely, lower CO2 levels can limit this process.

6. pH Levels of Soil:

Soil pH affects nutrient availability and can thus influence chlorophyll production. Most plants prefer slightly acidic to neutral pH levels (around 6.0 to 7.0). Extreme pH levels can inhibit nutrient absorption and negatively impact chlorophyll levels.

7. Plant Species and Genetics:

Different plant species inherently possess varying capacities for chlorophyll production based on their genetic makeup. For example, some plants naturally contain more chlorophyll than others, which affects their photosynthetic efficiency and growth patterns.

8. Environmental Stressors:

Factors such as pollution, herbicides, and pests can also adversely affect chlorophyll levels. Environmental stressors can lead to damage in the photosynthetic apparatus and impede the plant's ability to synthesize chlorophyll effectively.

Yosemitech offers two types of Chlorophyll Sensors: Online Chlorophyll Sensor and Self-cleaning Chlorophyll Sensor. The Online Chlorophyll Sensor is designed for continuous monitoring with high accuracy and a wide measurement range, making it ideal for various aquatic environments. On the other hand, the Self-cleaning Chlorophyll Sensor features an automatic cleaning mechanism that reduces maintenance needs, ensuring reliable and stable performance over time. Both sensors utilize advanced fluorescence technology to detect chlorophyll-a concentrations, providing valuable data for environmental monitoring, aquaculture, and research applications.

Measuring chlorophyll in water is essential for several reasons, primarily related to environmental monitoring, ecosystem health, and water management.

1. Indicator of Primary Production:

Chlorophyll is a crucial pigment found in phytoplankton, the microscopic plants in aquatic environments that perform photosynthesis. By measuring chlorophyll levels, we can gauge the primary productivity of a water body. Higher concentrations typically indicate a productive ecosystem with abundant phytoplankton, which forms the base of the aquatic food web.

2. Water Quality Assessment:

Chlorophyll levels are often linked to water quality. Elevated chlorophyll concentrations can signal nutrient pollution, particularly from nitrogen and phosphorus runoff, leading to algal blooms. Such blooms can deplete oxygen levels in the water, harming aquatic life and creating "dead zones." Monitoring chlorophyll helps identify and mitigate these pollution sources.

3. Ecosystem Health Monitoring:

Tracking chlorophyll is vital for assessing the health of aquatic ecosystems. Changes in chlorophyll concentration can indicate shifts in biodiversity, habitat quality, and the overall balance of the ecosystem. Regular monitoring allows researchers and policymakers to respond proactively to environmental changes, such as climate impacts or invasive species.

4. Algal Bloom Prediction:

Algal blooms can pose significant risks to human health, wildlife, and local economies. By measuring chlorophyll concentrations, scientists can predict and monitor blooms, allowing for timely warnings and management strategies. This is particularly important for water bodies used for drinking water, recreational activities, and fisheries.

From April 21th to 23rd, 2025, IE Expo China 2025 concluded successfully at the Shanghai New International Expo Centre. As a highly anticipated communication platform in the industry, the exhibition attracted exhibitors, experts, scholars, and industry elites from around the world to gather in Shanghai. Through various forms such as cutting - edge technology displays and high - end forum sharing, it created a professional and forward - looking audio - visual feast for the industry.

Suzhou Yosemite Technology Co., Ltd. ("YOSEMITECH"), which has been deeply involved in the water quality monitoring field for over 11 years, always adheres to the mission of "Water Monitoring Made Simple". YOSEMITECH showcased its self - developed water quality monitoring sensors and instruments at the exhibition. As a manufacturer of water quality monitoring sensors, we had in - depth exchanges with domestic and foreign partners and industry peers during the exhibition. It fully demonstrated its profound expertise in areas such as water quality sensor research and development and intelligent monitoring system integration, and continuously empowered global water quality monitoring scenarios with innovative technologies to promote the efficient and intelligent development of the industry.

Y514-A self-cleaning chlorophyll sensor calibration

Required equipments and raw materials
Required equipments: two 1L brown bottles, one iron stand, one set of gloves
Raw materials: 2mg/L chlorophyll standard solution and deionized water

Calibration steps
There are three methods of user calibration for the chlorophyll sensor: 0-point calibration, 1-point calibration and 2-points calibration.
0-point calibration was done in deionized water, 1-point calibration was done in 2mg/L chlorophyll standard solution, 2-points calibration was done in deionized water and 2mg/L chlorophyll standard solution.

0-point calibration step (change the B value)

Step 1: put an appropriate amount of distilled water or deionized water into a 1L brown bottle, remove the rubber protective cap on the front of the sensor and put the sensor in the center of the brown bottle, which not close to the wall around the brown bottle, and then fix the sensor with an iron stand, adjust the high and low position of the sensor. Pay attention to maintain a distance greater than 10cm between the front end of the sensor and the bottom of the brown bottle.
Step 2: Under the “Automatic Calibration” interface, click “0 point” to do zero-point calibration, wait for the values in the pop-up window to stabilize, and click “OK” to complete the calibration. Click “Get” to check whether the B value is written correctly.
Step 3: click “Start” under the “CHL” interface to get the real-time measurement of the CHL and temperature, check that the CHL is close to 0 to verify that the zero-point calibration is successful.

1-point calibration step (change the K value)

Step 1: put an appropriate amount of 2mg/L chlorophyll standard solution into a 1L brown bottle, remove the rubber protective cap on the front of the sensor and put the sensor in the center of the brown bottle, which not close to the wall around the brown bottle, and then fix the sensor with an iron stand, adjust the high and low position of the sensor. Pay attention to maintain a distance greater than 10cm between the front end of the sensor and the bottom of the brown bottle.
Step 2: Under the “Automatic Calibration” interface, change the “Single/First Point Calibration Value” to 227.1, click “1 point” to do one-point calibration, wait for the values in the pop-up window to stabilize, and click “OK” to complete the calibration. Click “Get” to check whether the K value is written correctly.
Step 3: click “Start” under the “CHL” interface to get the real-time measurement of the CHL and temperature, check that the CHL is close to 227.1 to verify that the one-point calibration is successful.

2-points calibration step (change the K and B values)

Step 1: put an appropriate amount of distilled water or deionized water into a 1L brown bottle, and put an appropriate amount of 2mg/L chlorophyll standard solution into another 1L brown bottle.
Step 2: remove the rubber protective cap on the front of the sensor, put the sensor in the center of the brown bottle containing distilled water or deionized water, which not close to the wall around the brown bottle, and then fix the sensor with an iron stand, adjust the high and low position of the sensor. Pay attention to maintain a distance greater than 10cm between the front end of the sensor and the bottom of the brown bottle.
Step 3: Under the “Automatic Calibration” interface, change the “Single/First Point Calibration Value” to 0, change the “Second Point Calibration Value” to 227.1, click “2 points” to do two-points calibration, wait for the values in the pop-up window to stabilize, and click “OK”. Then wipe the sensor dry and put it into 2mg/L chlorophyll standard solution according to the method of step 2, wait for the values in the pop-up window to stabilize, and click “OK” to complete the calibration. Click “Get” to check whether the K and B values are written correctly;
Step 4: click “Start” under the “CHL” interface to get the real-time measurement of the CHL and temperature, check that the CHL is close to 227.1, then clean the sensor with distilled water or deionized water, wipe it dry and put it into the distilled water or deionized water according to the method of step 2 to get the real-time measurement of the CHL and temperature, check that the CHL is close to 0 to verify that the two-points calibration is successful.